THE IMPACT OF IONIZED WATER SUPPLEMENTED WITH GLUTATION AND VITAMIN C DURING ACUTE HYPERTHERMIC EXPOSURE ON THE ACTIVITY OF THE SUPEROXID DISMUTASE IN THE BLOOD PLASMA, LIVER AND KIDNEYS AT WHITE LABORATORY RATS
Keywords:
Ionized water, hyperthermic stress, glutathione, vitamin C, superoxide dismutaseAbstract
Heat stress is among the most critical variables that might increase the generation of reactive oxygen species (ROS). Increased quantities of reactive oxygen and nitrogen species (ROS and RNS) have been shown to have negative consequences by affecting the cellular redox state. Several studies have shown that ionized water or Electrolyzed Reduced Water (ERW) can act as a scavenger free radical, which is produced by hydrogen molecules with a strong reducing capacity and may play a role in cellular redox regulation. The goal of our study is to see how ionized water affects the activity of superoxide dismutase (SOD) in blood plasma, liver, and kidneys during hyperthermic stress by adding enzymatic and non-enzymatic antioxidants, glutathione and vitamin C. White laboratory Wistar rats, female sex, weighing 180-220 g, young rats, separated into three groups of 15 individuals, were used in the experiment. Acute hyperthermic exposure at 41°C generated oxidative damage. The first group was given tap water as a control (KPM), the second was given ionized water (TAM), and the third was given ionized water with additional glutathione and vitamin C (TAD). The treatment lasted a total of 21 days. When compared to results obtained for the same enzyme activity on the 14th and 7th days of treatment, acute hyperthermic exposure on the 21st day significantly (p<0.001) reduced SOD activity in the KPM and TAM groups. The group treated with enhanced ionized water deviates from this general finding by showing a significant change in SOD activity between days 7 and 21, compared to a statistically significant difference (p<0.01) in the same group only from the 14th to the 21st day of treatment. In both groups KPM and TAD, the treatment given adequately during the period of hyperthermic exposure resulted in a substantial change in SOD enzyme activity in the blood plasma and liver. Acute hyperthermic exposure on day 21 did not result in a significant difference in renal SOD activity in the KPM and TAM groups.
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