THE EFFECT OF CIA ON SERUM IgG LEVELS IN WISTAR RATS

Abstract

Generally speaking, rheumatoid arthritis is a systemic disease followed by a complicated process of immune response and attack on the body's own organism, which results in inflammation, joint changes, development of vasculitis, changes in the nervous system and other systemic changes. Literature data indicate that rheumatoid arthritis is an autoimmune disease for which there is no cure, and therapy mainly consists of administering medications to relieve pain, improve the quality of life of patients and maintain working ability, with the extension of working life. Research and results obtained from experimental and clinical studies on the impact of RA on the human and animal organism, record changes that, among other things, occur in the biochemical parameters of serum samples. The subject of our interest in the paper are changes in the level of IgG in serum during arthritis. The main goal of our research was to induce RA using collagen type II in Wistar rats and to examine the differences in the parameters between the two groups of animals, the control and the treated group of male and female animals and to compare the results obtained between the sexes on the thirtieth and the sixtyth day after RA induction. For the research, eight week old white laboratory rats, male and female, were used, grouped into four groups: control 20 males, control 20 females, treated with collagen male 30 and treated female group 30 animals. The collagen for RA induction was prepared according to a specific protocol, and by pricking the right joint of the hind limb of the animals we introduced it into the body. We applied 100 μl of the collagen solution with 20 μl of physiological solution. Sera for analysis were obtained after centrifugation of the tubes, by decanting coagulated blood into monotubes. The IgG test was performed using a Dimension Max column chromatography apparatus. The IgG results in male animals in the first period have a significantly reduced IgG value. The IgG results in serum on the sixtieth day show that the level does not increase, but stagnates or decreases, but still remains close to the value on the thirtieth day after immunization. The IgG results in female animals have a significant decrease on the thirtieth day after treatment, compared to the control group. The IgG value on the sixtieth day remains almost the same as on the thirtieth day, and compared to the control group, there is a significant difference. The results obtained for male and female animals note the same dynamics of change in IgG levels in both sexes. It can be concluded that the tests of serum IgG levels in male and female experimental animals show a highly significant decrease in IgG values after collagen application, i.e. on the thirtieth day of treatment. It is characteristic that the reduced values are maintained in the second experimental period, i.e. on the 60th day. There is no statistically significant difference in the deviations in relation to the sex of the animals.