NITRITE REDUCTION IN PROCESSED MEAT PRODUCTS: PLANT-DERIVED NITRATE/NITRITE SOURCES, STARTER CULTURES AND HURDLE TECHNOLOGIES

Authors

  • Aleksandra Silovska-Nikolova Faculty of Agricultural Sciences and Food, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia

Keywords:

Nitrites, Nitrates, Plant-based sources, Starter cultures, Clean label

Abstract

Nitrites remain among the most important additives in processed meat products because they contribute simultaneously to cured colour development and stability, lipid oxidative protection, microbiological safety, and the formation of characteristic sensory attributes. However, growing concern about the potential formation of N-nitrosamines has intensified research into strategies to reduce nitrite use without compromising product quality and safety. This paper reviews current approaches to nitrite reduction in meat products, with particular emphasis on plant-derived nitrate and nitrite sources, nitrate-reducing starter cultures, and hurdle technologies as complementary tools within clean-label reformulation strategies. Particular attention is given to vegetables with high nitrate-accumulating potential, such as celery, parsley, spinach, chard, beetroot, radish, and Chinese cabbage, as well as to the importance of ingredient format, including juice, concentrate, powder, and fermented vegetable systems. The reviewed evidence indicates that such ingredients can provide a curing effect and, in some cases, quality characteristics comparable to those obtained with conventional nitrite addition. Nevertheless, their performance is strongly influenced by raw-material variability, agronomic and seasonal factors, processing conditions, and the efficiency of nitrate-to-nitrite conversion within the product matrix. The review also highlights the growing relevance of hurdle concepts, in which reduced nitrite levels are supported by synergistic combinations of plant ingredients, starter cultures, antioxidants, packaging systems, and non-thermal processes such as high-pressure processing, pulsed electric fields, cold plasma, and ultrasound-assisted treatments. Overall, nitrite reduction in processed meat products appears technologically feasible, but successful application depends on formulation standardisation, product-specific optimisation, and systematic monitoring of residual nitrite/nitrate levels, oxidative stability, microbiological status, and the possible formation of N-nitrosamines.

References

Alahakoon, A. U., Jayasena, D. D., Ramachandra, S., & Jo, C. (2015). Alternatives to nitrite in processed meat: Up to date. Trends in Food Science & Technology, 45(1), 37–49. https://doi.org/10.1016/j.tifs.2015.05.008

Andrade, B. F., Carmo, L. R. D., Tanaka, M. S., De Almeida Torres Filho, R., De Lemos Souza Ramos, A., & Ramos, E. M. (2025). Evaluation of nonthermal technologies to reduce or replace nitrite in meat products. Food Technology and Biotechnology, 63(1), 94-108. https://doi.org/10.17113/ftb.63.01.25.8744.

Bak, K. H., Bauer, S., Eisenreich, C., & Paulsen, P. (2025). Residual nitrite, nitrate, and volatile N-Nitrosamines in organic and conventional ham and salami products. Foods, 14(1), 112. https://doi.org/10.3390/foods1401011.

Bernardo, P., Patarata, L., Lorenzo, J. M., & Fraqueza, M. J. (2021). Nitrate Is Nitrate: The Status Quo of Using Nitrate through Vegetable Extracts in Meat Products. Foods, 10(12), 3019. https://doi.org/10.3390/foods10

Choi, Y., Kim, T., Jeon, K., Park, J., Kim, H., Hwang, K., & Kim, Y. (2017). Effects of Pre-Converted Nitrite from Red Beet and Ascorbic Acid on Quality Characteristics in Meat Emulsions. Korean Journal for Food Science of Animal Resources, 37(2), 288–296. https:// doi.org/10.5851/kosfa.2017.37.2.288.

Clayton-Cuch, D., Niklas, A. A., Duedahl-Olesen, L., Amlund, H., & Sloth, J. J. (2025). Nitrite, nitrate and N-nitrosamine occurrence in processed nitrate accumulating vegetables stored under different conditions. Food Chemistry, 493(Pt 1), 145631. https://doi.org/10.1016/j.foodchem.2025.145631.

Delgado-Pando, G., Ekonomou, S. I., Stratakos, A. C., & Pintado, T. (2021). Clean label alternatives in meat products. Foods, 10(7), 1615. https://doi.org/10.3390/foods10071615.

Dodocioiu, A. M., Buzatu, G., & Botu, M. (2025). Nitrates and nitrites in vegetables and the health risk. Foods, 14(17), 3037. https://doi.org/10.3390/foods14173037.

Eisinaitė, V., Vinauskienė, R., Viškelis, P., & Leskauskaitė, D. (2016). Effects of Freeze-Dried vegetable products on the technological process and the quality of dry fermented sausages. Journal of Food Science, 81(9), C2175–C2182. https://doi.org/10.1111/1750-3841.13.

Eisinaitė, V., Tamkutė, L., Vinauskienė, R., & Leskauskaitė, D. (2020). Freeze-dried celery as an indirect source of nitrate in cold-smoked sausages: Effect on safety and color formation. LWT, 129, 109586. https://doi.org/10.1016/j.lwt.2020.109586.

Golden, M. C., McDonnell, L. M., Sheehan, V., Sindelar, J. J., & Glass, K. A. (2014). Inhibition of Listeria monocytogenes in Deli-Style Turkey Breast Formulated with Cultured Celery Powder and/or Cultured Sugar–Vinegar Blend during Storage at 4°C. Journal of Food Protection,77 (10) , 1787–1793. https://doi.org/10.4315/0362-028x.jfp-14-059.

Gwak, S. H., Bae, S. M., & Jeong, J. Y. (2024). Evaluating the potential of vegetable powders as nitrite replacements in cured pork sausages: Effects of different processing methods to produce Chinese cabbage and radish powders. Food Science of Animal Resources, 44(5), 1028–1039. https://doi.org/10.5851/kosfa.2

e34.

Horsch, A., Sebranek, J.G., Dickson, J.S., Niebuhr, S.E., Larson, E.M., Lavieri, N.A., Ruther, B.L., Wilson, L.A. (2014). The effect of pH and nitrite concentration on the antimicrobial impact of celery juice concentrate compared with conventional sodium nitrite on Listeria monocytogenes. Meat Science. 96(1), 400-407. https://doi.org/10.1016/j.meatsci.2013.07.036.

Hosseini, M., Dezhangah, S., Esmi, F., Gharavi-Nakhjavani, M. S., Hashempour-Baltork, F., & Alizadeh, A. M. (2023). A worldwide systematic review, meta-analysis and meta-regression of nitrate and nitrite in vegetables and fruits. Ecotoxicology and Environmental Safety, 257,114934.https://doi.org/10.1016/j.ecoen

nv.2023.114934.

Hwang, K., Kim, T., Kim, H., Seo, D., Kim, Y., Jeon, K., & Choi, Y. (2018). Effect of natural pre-converted nitrite sources on color development in raw and cooked pork sausage. Asian-australasian Journal of Animal Sciences, 31(8), 1358–1365. https://doi.org/10.5713/ajas.17.0767.

Jin, S., Choi, J. S., Yang, H., Park, T., & Yim, D. (2018). Natural curing agents as nitrite alternatives and their effects on the physicochemical, microbiological properties and sensory evaluation of sausages during storage. Meat Science, 146, 34–40. https://doi.org/10.1016/j.meatsci.2018.07.032.

Kim, T.K, Lee, M.A., Sung, J. M,. Jeon, K. H., Kim, Y.B., Choi, Y.S. (2019a) Combination effects of nitrite from fermented spinach and sodium nitrite on quality characteristics of cured pork loin. Asian-australasian Journal of Animal Sciences 32(10), 1603-1610.

Kim, T.-K., Hwang, K.-E., Song, D.-H., Ham, Y.-K., Kim, Y.-B., Paik, H.-D., & Choi, Y.-S. (2019b). Effects of natural nitrite source from Swiss chard on quality characteristics of cured pork loin. Asian-Australasian Journal of Animal Sciences, 32(12), 1933–1941. https://doi.org/10.5713/ajas.19.0117.

Kim, M., Bae, S. M., Yoo, Y., Park, J., & Jeong, J. Y. (2025). Clean-Label Strategies for the replacement of nitrite, ascorbate, and phosphate in meat products: a review. Foods, 14(14), 2442.https://doi.org/10.3390/foods141

Kim, Y., Kim, T., Cha, J. Y., Kim, J., Park, M. K., Jung, S., & Choi, Y. (2025). Influence of naturally converted nitrite and ultrasound marination on the curing efficiency and residual nitrite of pork loin. Food Science of Animal Resources, 45(4), 1014–1026. https://doi.org/10.5851/kosfa.2024.e67.

Kos, I., Pleadin, J., Stvorić, M., Mirić, M., Širić, I., Lešić, T., Lazarus, M., Orct, T., Kljak, K., Stamenić, T., Ravlić, M., Jůzl, M., & Vnučec, I. (2025). Impact of incubation conditions and addition of red beet and leek powders as natural nitrate sources on the physicochemical and sensory properties of cooked sausages. Processes, 13(11), 3490. https://doi.org/10.3390/pr13113490.

Kureljušić, J., Maletić, J., Stanojević, S., Kureljušić, B., Petković, J., Vasić, A., & Bijelić, T. (2025). Nitrites in meat products in Serbia: harmful or safe? Foods, 14(3), 489. https://doi.org/10.3390/foods14030489

Lopez, C. M., Dallolio, G., Bonilauri, P., & Rebecchi, A. (2021). Strategies for Nitrite Replacement in Fermented Sausages and Effect of High Pressure Processing against Salmonella spp. and Listeria innocua. Foods, 10(11), 2617. https://doi.org/10.3390/foods10112617.

Luetic, S., Knezovic, Z., Jurcic, K., Majic, Z., Tripkovic, K., & Sutlovic, D. (2023). Leafy vegetable nitrite and nitrate content: potential health effects. Foods, 12(8), 1655. https://doi.org/10.3390/foods12081655.

Madentzidou, E., Gerasopoulos, D., Siomos, A. S., & Bloukas, I. (2012). Salt-stressed fresh cut leek accelerates CO2 and C2H4 production and enhances the development of quality characteristics of traditional Greek sausages during storage. Meat Science, 92(4), 789–794. https://doi.org/10.1016/j.meatsci.2012.07.002.

Magrinyà, N. M., Bou, R., Tres, A., Rius, N., Codony, R., & Guardiola, F. (2009). Effect of tocopherol Extract,Staphylococcus carnosusCulture, and celery concentrate addition on quality parameters of organic and conventional Dry-Cured sausages. Journal of Agricultural and Food Chemistry, 57(19), 8963–8972. https://doi.org/10.1021/jf901104h.

Marcinkowska-Lesiak, M., Alirezalu, K., Szpicer, A., Pogorzelska-Nowicka, E., Stelmasiak, A., & Poltorak, A. (2024). Optimizing nitrite content in powders from plasma-activated egg whites for meat preservation using response surface methodology. LWT, 208, 116736. https://doi.org/10.1016/j.lwt.2024.116736.

Molina, J. R. G., Melios, S., Crofton, E., Celayeta, J. M. F., Bolton, D. J., & Botinestean, C. (2025). Effects of nitrite level, packaging system and high-pressure processing treatment on physicochemical and sensory properties of cooked ham. LWT, 231, 118345. https://doi.org/10.1016/j.lwt.2025.118345.

Molina, J., Frías-Celayeta, J., Bolton, D., & Botinestean, C. (2024). Effects of natural colourants and pulsed electric field technology on the quality parameters of nitrite-free bacon. Food and Feed Research, 51(2), 211–218. https://doi.org/10.5937/ffr0-53899.

Munekata, P. E., Pateiro, M., Domínguez, R., Santos, E. M., & Lorenzo, J. M. (2021). Cruciferous vegetables as sources of nitrate in meat products. Current Opinion in Food Science, 38, 1–7. https://doi.org/10.1016/j.cofs.2020.10.021.

Ozaki, M. M., Munekata, P. E., Jacinto-Valderrama, R. A., Efraim, P., Pateiro, M., Lorenzo, J. M., & Pollonio, M. a. R. (2021). Beetroot and radish powders as natural nitrite source for fermented dry sausages. Meat Science, 171, 108275. https://doi.org/10.1016/j.meatsci.2020.108275.

Riel, G. L., Boulaaba, A., Popp, J., & Klein, G. (2017). Effects of parsley extract powder as an alternative for the direct addition of sodium nitrite in the production of mortadella-type sausages – Impact on microbiological, physicochemical and sensory aspects. Meat Science, 131, 166–175. https://doi.org/10.1 016/j.meatsci.2017.05.007.

Santamaria, P. (2006). Nitrate in vegetables: toxicity, content, intake and EC regulation. Journal of the Science of Food and Agriculture, 86(1), 10–17. https://doi.org/10.1002/jsfa.2351.

Shakil, M. H., Trisha, A. T., Rahman, M., Talukdar, S., Kobun, R., Huda, N., & Zzaman, W. (2022). Nitrites in cured meats, Health risk issues, Alternatives to Nitrites: A review. Foods, 11(21), 3355. https://doi.org/10.3390/foods11213355.

Sheng, S., Ricke, S. C., Silva, E. M., & Claus, J. R. (2025). Impact of organic and Conventional Vegetable‐Based Curing ingredients on Frankfurter quality and sensory attributes. Food Science & Nutrition, 13(4), e70148. https://doi.org/10.1002/fsn3.70148.

Silovska Nikolova, A., & Belichovska, D. (2020). Application of nitrites and nitrates as preservatives in processed meat production. Knowledge - International Journal, 38(3), 525–530.

Silovska Nikolova, A., & Belichovska, D. (2021). Application of natural sources of nitrite of plant origin in the meat processing industry. Knowledge - International Journal , 45(3), 577–582.

Silovska Nikolova, A., & Belichovska, D. (2022). Possibilities of nitrite replacement in meat products. Knowledge - International Journal, 51(3), 465–470.

Silovska Nikolova, A., & Belichovska, D. (2024). The effect of nitrite use in meat products on human health. Knowledge - International Journal , 63(3), 271–276.

Sindelar, J. J., Cordray, J. C., Sebranek, J. G., Love, J., & Ahn, D. U. (2007). Effects of varying levels of vegetable juice powder and incubation time on color, residual nitrate and nitrite, pigment, pH, and trained sensory attributes of Ready-to-Eat Uncured Ham. Journal of Food Science, 72(6), S388–S395. https://doi.org/10.1111/j.1750-3841.2007.00404.x.

Stoica, M., Antohi, V. M., Alexe, P., Ivan, A. S., Stanciu, S., Stoica, D., Zlati, M. L., & Stuparu-Cretu, M. (2022). New Strategies for the Total/Partial Replacement of Conventional Sodium Nitrite in Meat Products: a Review. Food and Bioprocess Technology, 15(3), 514–538. https://doi.org/10.1007/s11947-021-02744-6.

Sugita, K., Yamauchi, K., Ohashi, T., Suiko, M., Miura, M. (1993). Utilization of dried radish chip extracts in processing of sausages: An ingredient for a nitrite-free curing system. Nippon Shokuhin Kogyo Gakkaishi, 40(5) 339-347.

Xie, Y., Geng, Y., Yao, J., Ji, J., Chen, F., Xiao, J., Hu, X., & Ma, L. (2023). N-nitrosamines in processed meats: Exposure, formation and mitigation strategies. Journal of Agriculture and Food Research, 13, 100645. https://doi.org/10.1016/j.jafr.2023.100645

Zhang, Y., Zhang, Y., Zhang, Y., Zhang, Y., Jia, J., Peng, H., Qian, Q., Pan, Z., & Liu, D. (2023). Nitrite and nitrate in meat processing: Functions and alternatives. Current Research in Food Science, 6, 100470. https://doi.org/10.1016/j.crfs.2023.100470.

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Published

2026-06-03

How to Cite

Silovska-Nikolova, A. (2026). NITRITE REDUCTION IN PROCESSED MEAT PRODUCTS: PLANT-DERIVED NITRATE/NITRITE SOURCES, STARTER CULTURES AND HURDLE TECHNOLOGIES. KNOWLEDGE - International Journal , 76(3), 325–331. Retrieved from https://ojs.ikm.mk/index.php/kij/article/view/8320